RNA

Part:BBa_K5399000:Design

Designed by: josiazhang josiazhang   Group: iGEM24_NAIS   (2024-09-29)


sgRNA-SBEI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In plants, starch biosynthesis is a complex process that requires the synergistic action of various enzymes, including granule-bound starch synthase (GBSS), ADP-glucose pyrophosphorylase (ADPG), starch synthase (SS), starch branching enzymes (SBE) and starch debranching enzyme (DBE). Amylopectin is synthesized by the synergistic action of SS, SBE and DBE. The substrate required for the biosynthesis of both amylose and amylopectin is ADP- glucose, and there is substrate competition between them. During starch biosynthesis, SBE catalyzes the amylopectin α-1,6-glycosidic bond and participates in the formation of short chains within the amylopectin clusters, thus SBE plays an important role in the formation of amylopectin. SBE genes consist of two homologous genes, SBEI and SBEII. We plan to use the gene editing technology CRISPR/Cas9 to knock out both SBEI and SBEII in sweet potato to further increase the content of amylose in it.


Source

sgRNA-SBEI from Ipomoea batatas are synthesized.

References